Publications

Dr O’Kennedy has published extensively in the fields of mammalian cell culture and microbial based bioprocessing, Analytical techniques, PAT, QbD and cell line development.

Reprints for many of these publications are available on Researchgate.

 

Upstream Bioprocessing

  • O’Kennedy R.D. “ Multivariate analysis of biological additives for growth media and feeds” (2016) Bioprocess International 14(3):18-29
  • Kong, S., Rock, C., Booth, A., Willoughby, N., O’Kennedy, R., Relton, J., Ward, J., Hoare, M., Levy, M.S. (2008) “Large-scale plasmid DNA processing: evidence that cell harvesting and storage methods affect yield of supercoiled plasmid DNA.” Biotechnol Appl Biochem 51:43–51
  • Nealon,A.J., O’Kennedy, R.D., Titchener-Hooker,N.J. and Lye, G.J. (2006) “Quantification and prediction of jet macro-mixing times in static microwell plates.” Chem Eng Sci 61 : 4860-4870
  • O’Kennedy R.D and Steward P, “Bioreactor.” Patent number WO 2005/108549 (2005)
  • Nealon, A.J., Willson, K.E., Pickering,S.C.R., Clayton,T.M., O’Kennedy, R.D., Titchener-Hooker,N.J., and Lye,G.J., (2005) “Use of operating windows in the assessment of integrated robotic systems for the measurement of bioprocess kinetics.” Biotechnol Prog 21: 283-291
  • O’Kennedy, R.D., Ward, J.M. and Keshavarz-Moore,E. (2003) “Effects of fermentation strategy on the characteristics of plasmid DNA production.”  Biotechnol Appl Biochem 37 : 83-90
  • Tservistas, M., Levy, M. S., Lo-Yim, M. Y. A., O’Kennedy, R. D., York, P., and Hoare, M. (2001)  “The formation of plasmid DNA loaded pharmaceutical powders using supercritical fluid technology.” Biotechnol Bioeng 72 : 12-18
  • Levy, M.S., O’Kennedy, R.D., Ayazi-Shamlou, P., and Dunnill, P. (2000)  “Biochemical engineering approaches to the challenges of producing pure plasmid DNA.” Trends in Biotechnol 18 : 296-306
  • Brown, R.A., O’Kennedy, R.D., Helwigh,J., Madden, T., Hoare, M. (2000) “Accelerated definition of recombinant protein production in Saccharomyces cerevisiae using rapid monitoring techniques.” Enz Microb Tech 26 : 801-807
  • O’Kennedy, R.D., Baldwin,C., Keshavarz-Moore, E. (2000) “Effects of growth medium selection on plasmid DNA production and initial processing steps.” J Biotechnol 76 : 175-183

Bioprocess Physiology

  • Paoli, T., Faulkner,J., O’Kennedy, R., Keshavarz-Moore,E. (2010) “A study of D-lactate and extracellular methylglyoxal production in lactate re-utilising CHO cultures” Biotechnol Bioeng 107(1): 182-189.
  • Kong,S.,Day,A.F., O’Kennedy, R.D.,Shamlou,P.A.,Titchener-Hooker,N.J.(2009) “Using viscosity-time plots of Escherichia coli cells undergoing chemical lysis to measure the impact of physiological changes occurring during batch cell growth” J Chem Technol Biotechnol 10.1002/jctb.2101
  • Rozkov, A., Avignone-Rossa, C.A., Ertl, P.F., Jones, P., O’Kennedy, R.D., Smith, J.J., Dale, J.W. and Bushell, M.E. (2006) “Fed batch culture with declining specific growth rate for high-yielding production of a plasmid containing a gene therapy sequence in Escherichia coli DH1 cells.” Enz Microb Technol 39 : 47-50
  • Rozkov A., Avignone-Rossa C.A., Ertl P.F., Goryanin I.I., Jones P., O’Kennedy, R.D., Smith J.J., Dale J.W. and Bushell M.E. (2004) “Metabolic burden of acquisition of plasmids used for gene therapy on E. coli DH1 cells.” Biotechnol Bioeng 88:909-915
  • Kay, A., O’Kennedy, R.D., and Keshavarz-Moore,E. (2003)  “Impact of plasmid size on cellular oxygen demand in Escherichia coli” Biotechnol App Biochem 38 : 1-7
  • O’Kennedy, R. & Patching, J.W. (1997) “Effects of medium composition and nutrient limitation on loss of the recombinant plasmid pLG669-z by Saccharomyces cerevisiae.” J Ind Microbiol 18 : 381-387
  • O’Kennedy, R. & Patching, J.W. (1996) “Slow growth phenotype – A possible approach to improved plasmid maintenance in Saccharomyces cerevisiae.” Biotechnol Letts 18 : 713-718
  • O’Kennedy, R., Houghton, C. & Patching, J.W. (1995) “Effects of growth environment on recombinant plasmid stability in Saccharomyces cerevisiae grown in continuous culture.” Appl Microbiol Biotechnol 44 : 126-132

 

Analytical Techniques

  • Levy, M. S., Lotfian, P., O’Kennedy, R.D., Lo-Yim, M., and Ayazi-Shamlou, P. (2000)  “Quantitation of supercoiled DNA content during plasmid processing a fluorescence-based method.”  Nucleic Acids Research  28 (12): e57
  • Noites, I.S., O’Kennedy, R.D., Levy, M.S, Abidi, N., Keshavarz-Moore, E. (1999) “Rapid quantitation and monitoring of plasmid DNA using an ultrasensitive DNA binding dye.” Biotechnol Bioeng 66 : 195-201
  • O’Kennedy, R. & Patching, J.W. (1995) “Improved differentiation of ß-gal+ colonies on X-gal plates containing mycological peptone.” Biotechnol Tech 9 : 921-924

 

Process analytical technology

  • von Stosch, M., Davy, S., Francois, K., Galvanauskas, V., Hamelink, J.M., Luebbert, A., Mayer, M., Oliveira, R., O’Kennedy, R., Rice, P., and Glassey, J. (2014) “Hybrid modeling for quality by design and PAT-benefits and challenges of applications in biopharmaceutical industry” Biotechnol J. 9(6): 719-26.
  • Roychoudhury,P., O’Kennedy, R.D., Faulkner,F., McNeil, B., and M. Harvey,L.M.,(2013) “Chemometrics: Implementing multivariate data analysis to monitor mammalian cell culture processes” European Pharmaceutical Review 18(3): 15-20
  • Roychoudhury, P., Harvey L., McNeill B and O’Kennedy, R.D. (2008) “NIRS : a PAT tool for Biopharm applications.” Innov PharmaTechnol 25:62-65
  • O’Kennedy, R.D. (2008) “PAT for the product lifecycle of a biopharmaceutical product” American Pharmac Rev Jan-Feb Issue
  • Roychoudhury, P., O’Kennedy, R., McNeil, B,. Harvey, L.M.(2007) “Muliplexing fibre optic near infared (NIR) spectroscopy as an emerging technology to monitor industrial bioprocesses.” Analytical Chemica Acta 590 : 110-117
  • O’Kennedy R.D (2007) “PAT for the product lifecycle of a biopharmaceutical product” Euro Pharma Rev 1 : 73-82

 

Mathematical modelling and statistics

  • Flaherty, V., Mahony, T., O’Kennedy, R. & Colleran, E. (1998) “Effect of pH on the growth kinetics and sulphide toxicity thresholds of a range of methanogenic, syntrophic and sulphate-reducing bacteria.” Process Biochem 33 : 555-569
  • Dineen, T., Nolan, A., Harrington, J., Greer, A., O’Kennedy, R. & Houghton J.A. (1997) “Fluorescence in-situ hybridization studies on the sex chromosome constitution of human sperm.” Arch Andrology 39 : 217-222
  • Smith, P., Kane, M., Dempsey, S., Concannon, M., McDonagh, M., O’Kennedy, R., Patching, J.W., Callanan, K., Persell, L. (1996) “Theoretical and practical implications of minimum effect measurements of antimicrobial agents.” in  EuroResidue III : Residues of Veterinary Drugs in Food. Veldhoven, The Netherlands.
  • O’Kennedy, R., Camplisson, S. & Patching, J.W. (1994) “Microsoft® Excel macros for analysing plasmid instability and their application to recombinant strains of Saccharomyces cerevisiae.” Binary 6 : 204-210

 

Cell line development

  • O’Kennedy, R.D. & Patching, J.W. (1999) “The isolation of strains of Saccharomyces cerevisiae showing improved plasmid stability characteristics by means of selective continuous culture.” J Biotechnol 69 : 203-214